Part:BBa_K5178015:Design

T7RNAP_B0015

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2656
  Illegal NheI site found at 2660
  Illegal NheI site found at 2664
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2152
  Illegal XhoI site found at 471
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2585
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1074
  

Design Notes

We codon-optimized T7RNAP for B. subtilis.

Source

T7RNAP: https://www.ncbi.nlm.nih.gov/gene/?term=t7rnap